Preliminary establishment of in-house standards for the dried extract from ginseng cultivated in Vietnam

Abstract

This study was conducted to establish in-house standards for the dried extract from Vietnamese ginseng (Panax vietnamensis Ha et Grushv., Araliaceae). Radix and rhizoma of ginseng cultivated in Vietnam (VG), 6-year-old, were extracted by percolation extraction method.The in-house standards were then established for the final dried extract based on the Appendix 1.1, the Vietnamese Pharmacopoeia, fifth edition, and other reference standards. A set of standards of dried extract from VG was establishedthrough: organoleptic evaluation, loss on drying, total ash, ash insoluble in hydrochloric acid, pH, insoluble residue, microbial limit, the limit of heavy metals, qualitative and quantitative analysis. The quantitative standards were that in VG dried extract, the contents of ginsenoside-Rg₁ (G-Rg₁, C₄₂H₇₂O₁₄), majonoside-R2 (M-R2, C₄₁H₇₀O₁₄), ginsenoside-Rb₁ (G-Rb₁, C₅₄H₉₂O₂₃) were not less than 7.0%, 10.3%, 1.8%, respectively; the total contents of 3 main saponins were not less than 19.0%; the ratio of M-R2/G-Rg₁ were in the range of1.2-1.7%, calculated on the anhydrous basis.